A Classification Procedure for Functional RNA Crystal Structures
HD-RNAS is a classification of functional RNA structures available in PDB. All the RNA Crystallographic structures, NMR structures and Electron Microscopic structures having at least one RNA chain longer than 9 nucleotides are classified in 10 major functional classes. Each class is further classified according to the source organism from which the RNA molecules were isolated and crystallized.
Presently, HD-RNAS v2.0 contains 1060 crystal structures, containing 3066 RNA chains. The proper source organisms of RNA structure colected from sourse organism and other details field . The non-redundant set of structures consists of many PDB entries, indicating the best representative structures from each of the sub-classes based on their resolution and R-factor.
Structural/Functional Classification of 3066 RNA Chains
A Software for detection and analysis of Hydrogen Bonded Base Pairs in a nucleic acid structure. The program looks for at least two hydrogen bonds between two nucleotide bases. This can also detect possible protonated base pairs.
Citation : Das J., Mukherjee S., Mitra A., Bhattacharyya D. (2006). Non-canonical base pairs and higher order structures in nucleic acids crystal structure database analysis. J. Biomol. Struct. Dynam. 24, 149–161 [PubMed]
NUPARM
A Software for analysis of nucleic acid structure mainly through different base pair parameters.
Citation : Mukherjee S., Bansal M., Bhattacharyya D. (2006). Conformational specificity of non-canonical base pairs and higher order structures in nucleic acids: crystal structure database analysis. J. Comput.Aided Mol.Des. 20 (10-11), 629-645 [PubMed]
Frequently Asked Questions
Q1. I am using PDB ids like "1GTR.pdb, 1Y77.pdb " in ID Search but why it is not working? Ans. The ID search accepts pdb ids not pdb files. One should use ids like "1GTR, 1Y77". Do not use the ".pdb" part.
Q2. How to download in batch file? Ans. Press the Batch Download button first. Browse for file containing PDB id and Molecule id information in proper format. Click download button. The format should be like:
1GTR, 1, 2
3PIO, 1, 2, 3, 4, 5, 6
Tutorial
Search Database
One can search the database with respect to various parameters. Search related specific help are given in particular search types for more conveniance.
PDB/RCSB/NDB/BMRB ids can be used for search criteria. Multiple searches are catered while ids are seperated by comma (,). Use button after entering the id . Wrong typping can be cleared using the button Search Database > PDB/RCSB/NDB ids
Organism Search process is very similar to that of ID Search. In organism search one can simply query with Genus species orG. species type of query. Only one organism at a time can be search here.
Some well known functional RNA types are pre determined for Functional RNA Search catagory. One has to select the radio button on left of a particular catagory to search that. In case of rRNA/tRNA first select the radio button on left, then select specific type of rRNA/tRNA from dropdown on the right. Search Database > Functional RNA Types
RNA Sequences Search process tries to search full or part of RNA sequences within this database. No '>' notation or header regions are accepted, only sequences are to be given for search.
Crystal structures can be searched using time period criteria in Year Search. Molecules that are being deposited within this time period are shown in this process. Time period can be selected from pop-up calender or can be given in format of MM/DD/YYYY
Advanced Search
Database can be searched using various combination of search in Advance Search.
search criteria can be selected using dropdown and buttons with adds new criteria where removes the criteria
Non-redundant dataset can be searched using same criterias. One have to check the box on left of Search Non-redundant database only
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Authors: Debasish Mukherjee, Dhananjay Bhattacharyya
Last modified on: 15-Jul-2013 | For details of classification procedure click here | Please send your bug reports to: dhananjay.bhattacharyya[AT]saha.ac.in